BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube, Each. BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube. Remarks: Glucose – Any degree of yellow is positive. Acid end products from glucose fermentation turn the pH indicator from red (alkaline) to yellow (acid). of a Multitest System (Enterotube) for Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification . (ii) Modified lysine-lactose me- dium: a .. Bergey’s manual of determinative bacteriology.

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Reinsert wire without sterilizing into BBL Enterotube II, using a turning motion through all 12 compartments, until the notch on the wire is aligned with the opening of the tube Figure 3. The prepared reagent should have a straw yellow color and be stored in a brown bottle under refrigeration for maximum stability.

Media, incubation, and aseptic technique Objectives 1. Beige to light amber. This test detects the formation of pyruvic acid from the deamination of phenylalanine. American Society for Microbiology, Washington, D. With the exception of indole and VP, read the reactions in a sequential fashion by comparing the colors of the media in the tube after incubation with those given in the color scheme on the cover of the coding pad and eventually with an uninoculated BBL Enterotube II which must be brought to room temperature first Figure 5.

Identification of Unknown Organic Compounds Identification of Unknown Organic Compounds Introduction The identification and characterization of the structures of unknown substances are an important part of organic chemistry. A positive test is indicated by the development of a red color within 20 min. The discrepant biochemical test was resolved by use of a third method which gave a similar result as the BBL Enterotube II.

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Acetylmethylcarbinol acetoin is an intermediate in the production of butylene glycol from glucose fermentation. These chemical reactions make up the More information.

Enterotube ii computer coding manual – Google Docs

Information on the appearance of positive and negative reactions is provided in Table 1. Media, incubation, and aseptic technique Lab Exercise 3: Enzyme Pre-Lab Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab.

Bacterial isolation, identification and storage Chapter 1. A total of ten organisms were tested in duplicate over three days by three independent laboratories using the Enteeotube Enterotube II.

INSTRUCTIONS FOR USE BBL Enterotube II. IA Rev.: Jan. 2007

Materials – Work in groups of 4 per table. Any reagent having a dark brown color should not be used.

Pathogens virulence factors Yersinia pestis: This is a family of Gram-negative, oxidase-negative, facultative anaerobic rods. Practice microbial collection techniques Describe colony morphology and the relationship to microbial identification. Indigenous Unknowns Lab Report Introduction: It consists of several steps.

Amyl or Isoamyl Alcohol 75 ml p-dimethylaminobenzaldehyde 5 g Hydrochloric acid, concentrated 25 ml Dissolve p-dimethylaminobenzaldehyde the reagent should innterpretation light yellow in color in alcohol and then slowly interpretatioh the acid. Instruct students to put on lab coats and. Colony Morphology More information.

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Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth More information. Bacterial decarboxylation of lysine, which results in the formation of the alkaline end product cadaverine, is indicated by a change in the color of enterktube indicator in the medium from pale yellow acidic to purple alkaline.

This kit is stable More information. Where two or more organisms are listed, the confirmatory tests required to further identify them are also given.

On completion of this module, participants will be able to: Medical Microbiology Culture Media: It may be decided by the user ennterotube the databases with or without VP are used. A copper to brownish discoloration is read as negative. Energy of activation with and without an enzyme. This test is helpful in identifying and differentiating species of Bacillus, Clostridium, Proteus.

HTBC Number of experiments that enteroyube be performed: You will have 5 broth cultures at your workstation. Developing collaborative lab experiments across disciplines through the identification of bacteria Acknowledgements Developing collaborative lab experiments across disciplines through the identification of bacteria Joanna Huxster, Ph.

Serological testing is required for confirming Salmonella and Shigella spp. The following precautions pertain to the individual compartments indicated: